Journal: bioRxiv

Article Title: Macrophages foster adaptive anti-tumor immunity by ZEB1-dependent cytotoxic T cell chemoattraction

doi: 10.1101/2024.02.26.582102

Figure Lengend Snippet: A . Differentially expressed genes in unstimulated or LPS or IL-4 stimulated LysM ΔZeb1 compared to LysM Ctrl BMDMs as measured by a customized RT2 array and depicted in log 2 fold change of expression. nd marks non-detectable mRNA levels. All transcripts were normalized to Gapdh (n=3). B . Relative mRNA expression of Ccl2 and Ccl22 in LysM Ctrl and LysM ΔZeb1 BMDMs (n=3; means ±SD; 2-way ANOVA). C . Comparison of transcript and secretome alterations of Ccl2 and Ccl22 in LysM ΔZeb1 compared to LysM Ctrl BMDMs (n≥5; means ±SD).

Article Snippet: Final concentrations of cytokines were 1.93 ng/ ml CCL2 (R&D Systems, 479-JE-050) and 0.18 ng/ ml CCL22 (R&D Systems, 439-MD-025) and of antibodies 5 μg/ml IgG (Diagenode, C15410206), anti-CCL2 (Novus Biologicals, NBP1-07035SS) or anti-CCL22 (abcam, ab124768), respectively.

Techniques: Expressing, Comparison

Journal: bioRxiv

Article Title: Macrophages foster adaptive anti-tumor immunity by ZEB1-dependent cytotoxic T cell chemoattraction

doi: 10.1101/2024.02.26.582102

Figure Lengend Snippet: A-B . Venn diagrams of differentially expressed genes (DEGs) of LysM Ctrl (blue) and LysM ΔZeb1 BMDMs (red) after stimulation with LPS (A) or IL-4 (B) compared to unstimulated in total (left) and divided in up-/downregulated DEGs (right). C . GO term enrichment analysis for DEGs (FDR<0.05) uniquely up- or downregulated by LysM ΔZeb1 BMDMs after LPS stimulation. D . Log 2 fold change of expression of selected trafficking genes after LPS stimulation. X marks no significant deregulation. E . Representative images and quantification of OPP incorporation of LysM Ctrl and LysM ΔZeb1 BMDMs with 0h, 4h and 16h LPS pre-stimulation (n=3; means ±SD; 2-way ANOVA). F-G . Representative arrays of intracellular cytokines of LysM Ctrl and LysM ΔZeb1 BMDMs with LPS stimulation or additional Brefeldin A and Monensin treatment (F) and quantification of intracellular CCL2 and CCL22 after LPS, Brefeldin A and Monensin treatment (G; n≥2).

Article Snippet: Final concentrations of cytokines were 1.93 ng/ ml CCL2 (R&D Systems, 479-JE-050) and 0.18 ng/ ml CCL22 (R&D Systems, 439-MD-025) and of antibodies 5 μg/ml IgG (Diagenode, C15410206), anti-CCL2 (Novus Biologicals, NBP1-07035SS) or anti-CCL22 (abcam, ab124768), respectively.

Techniques: Expressing

Journal: bioRxiv

Article Title: Macrophages foster adaptive anti-tumor immunity by ZEB1-dependent cytotoxic T cell chemoattraction

doi: 10.1101/2024.02.26.582102

Figure Lengend Snippet: A . Confluence of KPC cells alone or co-cultured with LysM Ctrl or LysM ΔZeb1 BMDMs (n=3). B . Representative images at t=28h and quantification over time of KPC cell invasion into a scratch wound without or with co-culture of LysM Ctrl or LysM ΔZeb1 BMDMs (n=3). C . Confluence of KPC cells alone or with LysM Ctrl or LysM ΔZeb1 BMDM conditioned medium (CM) (n=2 KPC CM, n=3 LysM Ctrl and LysM ΔZeb1 CM). D . Transwell migration assay of CD8+ T cells alone or towards LysM Ctrl or LysM ΔZeb1 BMDMs in absence or presence of recombinant CCL2 and CCL22 (left panel, n>3) or absence or presence of anti-CCL2 and anti-CCL22 antibodies (right panel, n=3). Means ±SD; *:p<0.05; **:p<0.01; ns: not significant; 2-way ANOVA.

Article Snippet: Final concentrations of cytokines were 1.93 ng/ ml CCL2 (R&D Systems, 479-JE-050) and 0.18 ng/ ml CCL22 (R&D Systems, 439-MD-025) and of antibodies 5 μg/ml IgG (Diagenode, C15410206), anti-CCL2 (Novus Biologicals, NBP1-07035SS) or anti-CCL22 (abcam, ab124768), respectively.

Techniques: Cell Culture, Co-Culture Assay, Transwell Migration Assay, Recombinant

Journal: Shock

Article Title: Macrophage-Derived Chemokine (CCL22) Is a Novel Mediator of Lung Inflammation Following Hemorrhage and Resuscitation

doi: 10.1097/shk.0000000000000253

Figure Lengend Snippet: FIG. 4. Recombinant mouse MDC (rmMDC) exacerbates lung inflam- mation following hemorrhage and resuscitation (H/R). Representative micrographs of lung tissue stained with hematoxylin-eosin (AYC) and antiYmouse Ly-6B.2 (DYF) for inflammatory cell identification. The lungs from mice injected with rmMDC prior to resuscitation (C, F) had substantial inter- stitial tissue edema and cell infiltration as compared with sham mice (A, D) and mice resuscitated with LR solution only (B, E). G, Quantification of posi- tively stained cells confirmed the effect of rmMDC on increased inflammatory cell recruitment. **P G 0.001 vs. all groups and *P G 0.001 vs. sham.

Article Snippet: In separate studies, mice were hemorrhaged and injected intravenously with 1 2g of recombinant mouse MDC (R&D Systems) prior to resuscitation.

Techniques: Recombinant, Staining, Injection

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Pivotal role of the CC chemokine, macrophage-derived chemokine, in the innate immune response.

doi: 10.4049/jimmunol.164.10.5362

Figure Lengend Snippet: FIGURE 1. MDC protects mice from the lethality induced by CLP. The CLP mice were treated with i.p. injection of either MDC (1 mg/mouse; F; 15 mice) or vehicle (E; 15 mice) immediately after CLP. The survival rates were monitored for 7 days after CLP. Two different experiments were conducted, and the data were pooled. The mortality rates were very similar in individual experiments.

Article Snippet: Immediately after CLP, the mice were treated with either i.p or i.v. injection of recombinant murine MDC (1 mg/mouse; R&D Systems, Minneapolis, MN; endotoxin content , 0.1 ng/mg).

Techniques: Injection

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Pivotal role of the CC chemokine, macrophage-derived chemokine, in the innate immune response.

doi: 10.4049/jimmunol.164.10.5362

Figure Lengend Snippet: FIGURE 5. MDC blockade reduces survival of mice after CLP. The CLP mice were treated with i.p. injection of either anti-MDC antiserum (F; 30 mice) or control serum (E; 30 mice) 2 h before CLP. The survival rates were monitored for 7 days after CLP. Three different experiments were conducted, and the data were pooled. The mortality rates were very similar in individual experiments.

Article Snippet: Immediately after CLP, the mice were treated with either i.p or i.v. injection of recombinant murine MDC (1 mg/mouse; R&D Systems, Minneapolis, MN; endotoxin content , 0.1 ng/mg).

Techniques: Injection, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Pivotal role of the CC chemokine, macrophage-derived chemokine, in the innate immune response.

doi: 10.4049/jimmunol.164.10.5362

Figure Lengend Snippet: FIGURE 6. MDC induces respiratory burst in macrophages and lyso- zomal enzyme release from macrophages. Peritoneal cells were harvested from normal mice, the cells (1 3 106 cells) were incubated for 2 h at 37°C, and nonadherent cells were removed. A, The adherent macrophages were stimulated with murine MDC. After 30 min, the cultures were assayed for extracellular O2 2 release. B, The adherent macrophages were stimulated with murine MDC for 48 h at 37°C, and then the lysozyme enzyme ac- tivities in the culture supernatant were measured. The data are representa- tive of the three individual experiments (n 5 8 each). p, p , 0.05; ‡, p , 0.01; when compared with control (MDC 5 0 ng/ml).

Article Snippet: Immediately after CLP, the mice were treated with either i.p or i.v. injection of recombinant murine MDC (1 mg/mouse; R&D Systems, Minneapolis, MN; endotoxin content , 0.1 ng/mg).

Techniques: Incubation, Control